Equipment
The Fairlie Laboratories are equipped with, or have access to (within the IMB), a variety of state-of-the-art equipment both for synthesis and characterisation of small-molecules and peptides and for their evaluation in a range of biological systems.
1) NMR Spectroscopy. Adjacent to the synthesis laboratories on level 7 is an NMR facility, housing two 600 MHz Bruker NMR spectrometers with inverse probes (one 1H-13C, one 1H-X), one of which also has an LC-NMR probe and attached HPLC system available for use. These machines are typically booked a week or more in advance, whereas the Varian 400 MHz instrument is used as a walk-up machine for quick turnaround spectra and also has an autosampler for queuing samples overnight or during weekends. The 400 MHz spectrometer is equipped with a software-switchable quad-nucleus (1H, 13C, 19F, 31P) probe.
We also use a 900 MHz instrument on level 2 where there are 2 more NMR (500, 600 MHz) spectrometers which primarily serve the structural biology groups at IMB.
2x 600 MHz Bruker NMRs; Annika Yau using a 400 MHz Varian NMR; 900 MHz Bruker spectrometer.
2) Mass Spectrometry. The IMB houses a suite of Mass Spectometers. Our chemists typically use LCT and API2000 machines. Others are:
4700 MALDI-TOF-TOF
QSTAR Pulsar ESI-QqTOF
4000 QTRAP ESI-QqLIT
Voyager DE STR MALDI-TOF
Ciphergen SELDI-TOF
LCT ESI-TOF
API 2000 ESI-QqQ triple quadrupole
ZMD single quadrupole
Bruker MicroTOF for high-resolution MS
Dr Gloria Ruiz-Gomez measuring mass spectra on Micromass LCT and Bruker MicroTOF mass spectrometers
4700 MALDI TOF-TOF and nano HPLC ESI QqTOF, Quadrupole Time of Flight QSTAR Elite Mass Spectrometers
nano/micro HPLC ESI QqQ (LIT), Quadrupole LInear Ion Trap, 4000 QTRAP and analytical HPLC ESI QqQ, Triple Quadrupole, API2000 Mass Spectrometers
3) HPLC equipment. Waters Analytical, semi-preparative and preparative (Deltaprep) systems, Agilent Analytical, automated semiprep, and LC-MS systems.
4) Freezer driers and Biotage and CEM Microwave reactors.
5) Parallel synthesiser and purification system (ideal for focused library production)
Carousel 12 Plus Reaction Station and VacMaster-10 & 20 Sample Processing Manifolds
Radleys Greenhouse Blowdown Evaporator
Biotage Isolera Four - the high performance FLASH purification system
6) Circular Dichroism, Ultraviolet, and Infrared Spectrometers
Jasco CD spectrometer
Jasco Infrared spectrometer
Varian Cary UV spectrophotometer
7) Molecular modelling facilities. We use a dedicated molecular modelling room which houses Linux and SGI workstations. Software in use includes the Sybyl suite of programs from Tripos, InsightII, modeller, Pymol, CNS, Xplor-NIH, the openeyes suite (inc. Omega2, ROCS, EON, FRED), Gold, Dock and Autodock for docking requirements. Additional shared access workstations (Mac, Linux, Windows) throughout the office area can access this software remotely or in some cases native MacOSX and Windows versions are available. Larger parallel modelling jobs such as large scale docking of our 50 million compound databases, molecular dynamics, and conformer generation/searching are usually sent to the shared IMB cluster infrastructure. There is also located in the IMB in shared modelling and bioinformatics space on level 6 West.
Dr Andrew Lucke and Dr Praveen Madala at computer modelling machines, middle pictures are the server and 40 cpu cluster facility.
8) Cell Biology
BMG Fluostar Optima
Flow cytometry: BD FACSCantoII flow cytometer (with 8 colours) and
FACSCalibur system
Confocal microscopes: 2 Zeiss LSM-510 META inverted microscope (1 with
blue laser diode system, 1 is 2-photon capable)
Fluorescence microscopes: 3 Olympus BX-51 and 1 Olympus BX-60
FRET study: Olympus AX-70 for FLIM and FRET
Real time imaging: Olympus IX-81 OBS real time
BD Pathway Bioimagers
Perkin Elmer EnVision Multilabel plate reader
Real time PCR: 2 ABI Prism 7000, 2 ABI 7500 and 1 ABI 7900.
Tissue culture facilities
In house microarray support facility
L, C - Zeiss LSM-510 META inverted microscope (1 with blue laser diode system, 1 is 2-photon capable), R - BD FACSCantoII flow cytometer (with 8 colours).
